Prof.Dr. Erhan Pişkin
Engineering of Bone and Cartilage Tissues
Tissue engineering is one of the recent theraputic approaches for both soft and hard tissue repair. Healthy cells taken from the host own tissues or from other sources are used together with scaffolds. Target specific (eg., osteoblasts, chodrocytes, etc.) or preferentially stem cells are isolated, differentiated (in the case of stem cells), or even genetically modified (for instance to express growth factors, eg., BMPs) and are used in two diffetrent approaches. In the first approach they are just loaded into the scaffolds (as seeds) and apply as biohybrid implants. Alternatively, cells are propagated within the pores of scaffolds within bioreactors (in vitro) to form tissue-like structures and then they are implanted for tissue replacement. Scaffolds have large and interconnected pores which allows 3D-cell ingrowth are used. They have to be degradable in vivo, means that they should degrade such a rate that the new forming tissues to replace them properly. Of course both they and their degradation products must be biocompatible. They are produced several techniques, such as moulding/salt extraction, electrospinning, cryogelation, etc. They are made of several natural polymers (e.g., collagen and its denaturated form gelatin) and synthetic polymers (e.g., lactides, glycolide and ε-caprolactone). Several bioactive agents (e.g., growth factors, etc.) may be also incorporated (usually as controlled release formulations) to trigger the regeneration rate and proper new tissue formation. After careful in vitro biocompatibility test, tissue engineering scaffolds (loaded with cells) or biohybrid implants are applied in vivo in proper animal models. Critical size defects (means that the defects do not recover by themselves) are created in animals. In the maxillofacial applications, cranium, cleft palate, zgyoma, mandibula, etc. models have been used for bone tissue engineering. Ear defects are created to study cartilage repair. Several macro-, histological, molecular techniques are used to investigate tissue regeneration. This talk briefly reviews the topics mentioned above by using the experience of the author’s group in this field.
Prof. Erhan Pişkin was graduated from Ankara University, Chemical Engineering Department in 1970, and joined Hacettepe University, Chemical Engineering Department. He completed his PhD in January 1976 at Hacettepe University, became and Associated Professor in 1981 and then a Full Professor in 1988. He is the founder of “Bioengineering Graduate Program” as a Division at Institute of Science, Hacettepe University. He is the founder and now director of NanoBMT: Nanobiyomedtek Biyomedikal ve Biyoteknoloji San.Tic. Ltd.Sti – a SME devoted to only R&D activities, since 2014. He was a Member of “Science Board” of Turkish Scientific and Technological Council for about 9 years (2000-2009). His awards include TÜBİTAK-Junior Scientist Award (1981); C.P. Sharma International Award in Biomedical Engineering (1997); Hacettepe University-Science Award (1999); and TÜBİTAK-Science Award (2000). He has about 362 scientific papers in internationally cited journals + about 20 chapters in books + more than 250 presentations in international and national meetings + editor/author of 10 Books (6 in English and 4 in Turkish). His citations are close to 6000 with an HI index of a 41. He has supervised more than 50 PhD and 100 MSc theses in the last 30 years. He has organized about 20 International and Several National meetings in Turkey. His main research interests are: polymers: synthesis and use in diverse fields, mostly as biomaterials (biodegradable) and Tissue Engineering and Regenerative Medicine (recently with stem cells); gene transfer with nonviral polymeric vectors; Nanoparticles and structures for biosensors EIS, SEFS, SERS, MALDI-TOF, etc. and array technologies recently for detection of pathogenic bacteria using bacteriophages; bioaffinity separation, diagnostic test kits and drug delivery based on polymeric carriers.
Honorary Member of Turkish Academy of Sciences
Emeritus Professor from Hacettepe University, Ankara, Turkey
Visiting Professor at Mugla Sıtkı Kocman University, Resaerch Labs Center, Mugla, Turkey
Manager/Owner of NanoBMT: Nanobiyomedtek Biyomedikal ve Biyoteknoloji San.Tic. Ltd.Şti.,